The HER2 SISH assay was carried out on the seven primary gastric adenocarcinomas that scored IHC 2+ and on the 10 CB-MEs that scored IHC 1+ to 3+, but amplified HER2 was found only in the three CB-MEs that scored IHC 3 + . The gene discussed is ERBB2; the disease is gastric adenocarcinoma.