Using a cut-off of 1.06 for the DNA integrity index, a cut-off of 12.91 nM for pleural fluid Mesothelin and a cut-off of 1.34 nM for serum Mesothelin, the authors obtained a specificity of 90% and a sensitivity of 75% to distinguish malignant pleural mesothelioma from benign pleural effusion. Here, MSLN is linked to malignant pleural mesothelioma.