We wanted to explore the NRF2-NQO1 axis by inducing stress through incubating cancer cell lines in the following: A medium with a decreased concentration of glucose (1 g/L) (NC2); a medium with a complete lack of glucose (NC4) but supplemented with glutamine; and, in order to induce the strongest level of ROS, by creating an artificial situation, using a medium deprived of glutamine and glucose (NC3). The gene discussed is NFE2L2; the disease is cancer.