Finally, the efficacy of 10_12-CL4 was also tested on SK-BR-3 cells co-cultured with human lymphocytes to exploit also the inhibitory effects of 10_12 mAb in the PD-1/PD-L1 interaction [15,59] between tumor cells and T cells, which activates T cells by inducing IL2 and IFNγ cytokines secretion, as reported above and further detailed in another manuscript [55]. This evidence concerns the gene CD274 and neoplasm.