To corroborate the involvement of the endosomal sorting complex required for transport (ESCRT) in CD40L synaptic transfer to BSLB we targeted tumour susceptibility gene 101 (TSG101) and vacuolar protein sorting 4b (VPS4b) using clustered regularly interspaced short palindromic repeats (CRISPR) gene editing in primary human T cells. Here, TSG101 is linked to neoplasm.