In a study performed by Vassilatou [36] Androgen Excess PCOS (AE-PCOS) criteria were used and demonstrated the predictors as FAI and a decrease in SHBG and HOMA-IR, but the same researchers in 2018 identified VAI as a predictive factor for PCOS diagnosis after using the Rotterdam criteria [34]. This evidence concerns the gene SHBG and polycystic ovary syndrome.