We identified six novel UGT1A1-associated variants in our hyperbilirubinemia patients, including four missense variants, one nonsense variant, and one splicing variant. In silico analysis using SIFT, Polyphen-2, and MutationTaster [24–26] predicted the variants of p.Asp259Glu, p.Glu463Lys, and p.Val491Met as being likely pathogenic while p.Ile268Val was predicted as benign (data not shown). This evidence concerns the gene UGT1A1 and Hyperbilirubinemia.