In this context, the major aim of the present study was to examine the effect of a zinc metalloprotease derived from Bothrops moojeni snake venom (BmooMP-alpha-I) as a potential therapeutic agent to treat intestinal inflammation in a murine model of colitis induced by dextran sulfate sodium, considering that this enzyme can interfere in the TNF biological function by directly promoting its hydrolysis. The gene discussed is TNF; the disease is colitis.