qPCR analysis of NCLX mRNA expression found ~75% loss of NCLX in the frontal cortex of 2-month-old NCLX-cKO × 3xTg-AD mice (Fig. 2b) and a corresponding reduction in hippocampal NCLX protein expression in 2, 9, and 12-month-old mice (Fig. 2c; Supplementary Fig. 7N–P). The gene discussed is SLC8B1; the disease is Alzheimer disease.