Although phosphorylation was found to be elevated at all the tau residues examined in 3xTg-AD brains, we only found differential phosphorylation with the AT8 antibody (tau S202/T205) in addition to a significant increase in the total levels of insoluble tau in NCLX-cKO × 3xTg-AD brain homogenates (Fig. 2n; Supplementary Fig. 7X–C’). This evidence concerns the gene MAPT and Alzheimer disease.