The resultant neuronal-specific NCLX conditional knockouts (NCLXfl/fl × Camk2a-Cre, NCLX-cKO) were backcrossed into the 3xTg-AD mutant mouse background (NCLX-cKO mice × 3xTg-AD, Fig. 2a). This evidence concerns the gene CAMK2A and Alzheimer disease.