Next, we generated ApoEVs and ApoEVs-HM from the melanoma cell line SK-Mel28 according to the protocol described in Figure 1A. The SK-Mel28-derived ApoEVs-HM also contained DC-SIGN-binding ligands as detected with DC-SIGN-Fc ELISA (Figure S3), demonstrating that this method can be applied to different tumor cell lines for the generation of DC-SIGN-binding ApoEVs. Here, CD209 is linked to melanoma.