The sorted (CD133+ and CD133−) and unsorted NSCLC cell lines were co-cultured with MSCs (MSC-TRAIL or MSC-EV) at different NSCLC-cell-to-MSC ratios (1:1, 1:3, and 1:6) in a 96-well tissue culture plate for 72 h, and rhTRAIL was used as a positive control for the treatment. Here, TNFSF10 is linked to non-small cell lung carcinoma.