To gain clinical relevance of the above findings and to compare the prognostic value of different ASPM isoforms, we examined the tumor tissues for the staining patterns of ASPM‐iI, ASPM‐iII, active β‐catenin (a marker of activated Wnt–β‐catenin signaling), and the stemness marker ALDH‐1, which has been shown to be prognostically important in PDAC 12, at the single‐cell resolution in a cohort of 50 consecutive PDAC patients who received surgical resection of their primary tumors (supplementary material, Table S1). Here, ASPM is linked to neoplasm.