E-selectin ligands expressed by (A) U937 monoblasts, (B) PSGL-1, CD43, CD44 and CLA positive myeloblasts from 2 patients with AML (AML#85 and #87) and (C) lymphoblasts from a patient with a PSGL-1 negative, CD43, CD44 and CLA positive B-ALL (ALL#21) were adsorbed separately from blast lysates on protein G-Sepharose beads coated with E-selectin/μ chimera, separated under non-reducing conditions by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and revealed by western blotting. The gene discussed is SELPLG; the disease is acute lymphoblastic leukemia.