After infection and adenoviral expression of AC9-D399A for 48 h, cells were stimulated with 1 μM isoproterenol for 5 min and then assayed by immunoprecipitation of Yotiao followed by western blotting for PKA phosphorylation of Ser-27 of KCNQ1 versus total KCNQ1 (Figure 5). This evidence concerns the gene KCNQ1 and infection.