Here, we extend these studies to show for the first time that infection by all 4 DENV serotypes impacts on PML-NB structure, establishing that of the nuclear localizing DENV proteins, NS5 proteins but not NS3 or C are responsible, and that PML isoforms III and IV, but not other PML isoforms or PML-NB components, are the targets of NS5 action. This evidence concerns the gene RAF1 and infection.