In addition, a recent study demonstrated that cytosine DNA methylation which acts as a transcriptional “ON-OFF” switch and a specific over-expression of tubulin beta 2B class IIB (Tubb2b) mediated by aberrant DNA methylation are closely associated in the development of NASH-HCC in a mouse model (48). This evidence concerns the gene TUBB2B and metabolic dysfunction-associated steatohepatitis.