RUNX1 and acute myeloid leukemia: Microscopic examination may be used to detect distinctive features (e.g. Auer rods) in cell morphology, cytogenetic analysis to identify chromosomal structural aberrations (e.g., t(8;21), inv(16), t(16;16), or t(9;11)), and molecular genetic analysis to identify gene fusion (e.g., RUNX1-RUNX1T1 and CBFB-MYH11), and mutations in genes frequently mutated in AML (e.g., NPM1, CEBPA, RUNX1, FLT3)1,3,5,10–12.