Using control MGH7 cells, a lung squamous cell carcinoma cell line with high levels of NRF229,30 (Fig. 4A), we identified a lentiviral shRNA construct (shRNA2) that was especially effective at reducing protein levels of NRF2 and the NRF2 target gene, NQO1 (Fig. 4A,B). The gene discussed is NFE2L2; the disease is squamous cell lung carcinoma.