Similarly, GC B cell (B220+PNA+FAS+) response in immune-modulated ZIKV infection followed the same trend as the Tfh and pre-Tfh cell responses, with a peak response of 12.7% of total B cells on 7 dpi, and then declined on days 14 and 28 (Supplementary Fig. 2), kinetically consistent with the rapid elicitation of nAb responses (Fig. 1a, b). Here, FAS is linked to Zika virus infectious disease.