Our RNA-seq analysis revealed that many genes involved in IL-12-stat4, and IFN-γ pathways were upregulated in Th1-like Tfh cells (Fig. 3), suggesting that CD4+ T cells may receive more signals from IL-12 or IFN-γ pathways provided by dendritic cells (DC) during Tfh cell differentiation in ZIKV infection. This evidence concerns the gene CD4 and Zika virus infectious disease.