A total of 100 enterococci isolated from urine samples of hospitalized patients with symptomatic urinary tract infections were investigated for antimicrobial susceptibility, the frequency of aminoglycoside and vancomycin resistance genes (including aac (6′)-Ie-aph (2“)-Ia, aph (3’)-IIIa, ant (4’)-Ia, aph (2”)-Ic, aph (2“)-Ib, aph (2”)-Id, ant (3′′)-III, ant (6′)-Ia, vanA, vanB and vanC) and virulence encoding genes (including gelE, PAI, esp, ace, cyl, hyl and sprE). Here, ACE is linked to urinary tract infection.