The additional reduction in glutaminase enzyme (GLS1,2) and glutamine transporter (ASCT2) induced by the HLA-B,C-specific mAb B1.23.2 in melanoma cells, may reduce many of the building blocks derived from tri-carboxylic acid cycle (TCA) intermediates that need to be replenished in a process called anapleurosis. The gene discussed is SLC1A5; the disease is melanoma.