The obtained MRM chromatograms for the analyzed urine sediment samples originating from animal with diagnosed MMVD (Figure 2) and CKD (Figure 3) show peaks corresponding to the transition characteristic for the selected canine tryptic podocin peptide with the 218H-AAEILAATPAAVQLR-OH232 sequence with the confirmed retention time. Here, NPHS2 is linked to chronic kidney disease.