Finally, we further evaluated the distribution of specific markers in sEVs produced by the different GBM cells, including fibronectin, CD63, HSP70, Annexin A2, CD9 CD81, as well as CD44 which has been recently observed at the surface of EVs from different sources, such as ovarian and breast cancer cells but also mesenchymal stem cells, whilst being associated with GBM progression and aggressiveness23,29,35–37. This evidence concerns the gene CD81 and glioblastoma.