ITSN1 and Tako-tsubo cardiomyopathy: Furthermore, in ASC case-control samples,7 LGD variants in ITSN1 were also identified in the controls (three in 5397 ASC controls and comparable with the cumulative AF of 2.5e−4 in gnomAD v2.1), although they were still overrepresented in cases (two in 1601 cases).