Finally, to evaluate whether KRAS mutational status affects ORP5 and ORP8 basal levels, we used the isogenic colorectal cancer cell line HCT116; the parental line harbors a heterozygous KRAS mutation, whereas its derivative line has a single WT KRAS allele after KO of the mutant allele via homologous recombination (Markowitz et al, 2009). The gene discussed is OSBPL8; the disease is colorectal cancer.