In this respect, the principal aim of our study was to investigate the influence of primary hypertension on SKA-31-mediated systemic hemodynamic effects in anesthetized rats, and also to investigate putative endothelium-dependent mechanisms, including EDH-KCa2.3/KCa3.1 type relaxation in isolated endothelium-intact small mesenteric arteries (sMAs). The gene discussed is KCNN4; the disease is essential hypertension.