ERVW-1 and infection: To measure 50% inhibitory concentration (IC50) of 2-bromoaldisine to inhibit HIV-1 Env-mediated infection, HeLaCD4 cells were pretreated with 2-bromoaldisine for 5 h at various concentrations, washed with PBS, and inoculated with luciferase-encoding, HIV-1 Env-carrying HIV-1 vector in a six-well plate.