Furthermore, the activation of TLR2 and TLR4 are found to be related with gingivitis and periodontitis in vivo (Yoshioka et al., 2008; Ilango et al., 2016), in line with our study where we found that the gingivitis biofilm induced more upregulation of TLR4 transcription compared to commensal and cariogenic biofilms. This evidence concerns the gene TLR4 and gingivitis.