Since we showed that infection with pRBCs, and thus parasite GPI, induced GPI specific MBC activation in previously vaccinated mice compared to baseline (Figure 3IIIC) and naïve mice (Figures 4IIA,B) we sought to investigate if Plasmodium mice underwent GPI-specific MBC reactivation when challenged with synthetic GPI-KLH or a third infection with pRBCs (Figures 4IIIA,B). This evidence concerns the gene GPI and infection.