Moreover, GSEA also confirmed the prognostic nature of AK4-AK1 gene signature as nearly 50% of the genes in the gene signature were enriched in lung cancer poor survival gene sets reported by Director’s Challenge Consortium for the Molecular Classification of Lung Adenocarcinoma (Fig. 4b and Supplementary Table S3)29. This evidence concerns the gene AK1 and lung adenocarcinoma.