ITCH and cancer: To determine whether ITCH-induced c-FLIPL degradation had a major role in anti-HER3 antibody-induced cancer cell apoptosis, we assessed PARP and caspase-8/3 cleavage by western blotting in siITCH and siSC BxPC3 cells incubated with 9F7-F11 alone (Fig. 5a) or with NRG1 (Fig. 5b) for 48 h.