PRKAA2 and hydrops fetalis: Immunostaining of phosphorylated AMPKα2 in the glomeruli and renal tubules indicated intense staining in the ND/ND group (150 ± 15 positive cells), an intermediate level of staining in the HF/ND group (72 ± 11 positive cells) and ND/HF group (85 ± 21 positive cells), and little staining in the HF/HF group (24 ± 17 positive cells) (Figure 3B).