On one hand, in present study, obesity induced by high-fat diet down-regulated plasma GnRH, FSH, LH, and T levels, while it up-regulated E2 levels compared to the control group, as Vigueras-Villaseñor R M et al. [61] reported in a similar experiment. Here, GNRH1 is linked to obesity due to melanocortin 4 receptor deficiency.