To establish suitable cell lines for the analysis of BCL9 and BCL9L expression, we tested six HCC cell lines (HLE, HLF, Huh7, HepG2, Hep3B, and Huh6) and two normal liver cell lines (THLE-2 and THLE-3) for BCL9, BCL9L, and β-catenin protein expression (Fig. 1b). This evidence concerns the gene BCL9L and hepatocellular carcinoma.