At the same time, Gal3kd alleviated MCT-induced Pulmonary vascular structural changes by reduced media wall thickness and increase non-muscularized percent of vessels, Gal3OE dramatically increased medial wall thickness as well as percent of occlusion lesion vassels (Fig. 2F and 2G) which suggesting Gal-3 knockdown could prevent PAH development by reversed vascular change and inhibit followed hemodynamic disorder and right ventricular hypertrophy. This evidence concerns the gene LGALS3 and pulmonary arterial hypertension.