To quantify the extent of EndoMT in each PAH model in vitro, and to evaluate the significance of Gal-3 in EndoMT and its relative markers, VE-cadherin, αSMA and TGFβ1 (predominant profibrotic signal in EndoMT) were assessed in each cell model. Here, TGFB1 is linked to pulmonary arterial hypertension.