By statistical analysis, we found the somatic mutations were significantly enriched in the regions around the calpain cleavage sites compared with other regions, while the μ-calpain and m-calpain mRNA expression levels were differentially expressed across 11 cancer types, which was consistent with previous analyses (Kimura et al., 1998; Lakshmikuttyamma et al., 2004; Storr et al., 2011; Storr et al., 2012). This evidence concerns the gene CAPN2 and cancer.