TNF and infection: For further clarification, we identified changes in the expression of TNF-α, IL-12p70, and IL-10 in MAH-infected DCs co-infected with P. aeruginosa, and in particular, the expression of IL-10 dramatically increased, confirming that TLR4 signaling in MAH infected cells showed the same result as in the actual infection with Gram-negative bacteria (Supplementary Figure S1).