To clarify the mechanisms, we compared the gene expression profiles of the tumours of the ND-fed PyMT mice and those of the HFD-fed PyMT mice by performing quantitative RT-PCR (qPCR), but the results showed no significant differences in the expression of macrophage markers (F4/80, CD11b, CD11c, CD206, and CD163), proinflammatory cytokines (Il1b, Il6, Il10, and Ifng), angiogenic markers (Igf1, Hgf, Angpt1, Angpt2, Tie2, and Vegfa), myeloid chemoattractants (Ccl2 and Ccl7), Leptin, or Lepr between the two groups (Fig. 1c). This evidence concerns the gene CD163 and neoplasm.