Because the results showed that the HFD affected neither the Opn expression levels in F4/80+ macrophages nor the proportions of CD11c+ M1-like macrophages and CD206+ M2-like macrophages in the tumours, the marked induction of Opn expression in the HFD-fed PyMT mouse tumours (Fig. 1c) must be attributed to other cells, not macrophages. The gene discussed is SPP1; the disease is neoplasm.