Notably, culturing these DIPG cell lines in growth media devoid of nicotinic acid (NA) induced a strong sensitivity to FK866 in all SU-DIPG spheroid cultures tested (Supplementary Fig. 4c), confirming the importance of alternative NAD biosynthesis pathways such as NA salvage, in mediating NAMPT inhibitor synthetic lethality in gliomas. Here, NAMPT is linked to central nervous system cancer.