To ascertain if this effect was specifically limited to DIPG and astrocyte models, we validated our results in the osteosarcoma cell line, U2OS (R458fs), as well as the breast cancer cell line MCF7 (PPM1D amplification), both which contain endogenous PPM1D alterations (Supplementary Fig. 5a, b). Here, PPM1D is linked to osteosarcoma.