Using established procedures17,19, we generated organoid cultures from the pancreata of wild-type C57Bl/6 mice (n = 3) and from pancreatic tissues of KPC (Kras+/LSL-G12D; Trp53+/LSL-R172H; Pdx1-Cre)16 mice (n = 3), which contained invasive PDA (Fig. S1a). This evidence concerns the gene TP53 and Patent ductus arteriosus.