We also conducted a cellular phosphorylation assay of CSF-1R and the downstream signaling molecules Erk and Akt using a CSF-1-dependent murine myelogenous leukemia cell line (M-NFS-60) and CSF-1-differentiated mouse BMDMs treated with 3D185 or PLX3397. Here, AKT1 is linked to myeloid leukemia.