TNF and neoplasm: Notably, treatment with 3D185 also resulted in a decrease in T regulatory cells (Fig. 6f), the tumor-promoting subpopulation of CD4+ T cells, and a significant increase in the infiltration of activated CD8+ T cells (IFN-γ+ CD8+ T cells, TNF-α+ CD8+ T cells), though it did not affect the CD8+ T cells infiltration ((Fig. 6g, h, Additional file 1: Figure S5F).