Concentrations of some peptidases, proteases and proteasome associated enzymes (e.g. methionine aminopeptidase 2, calpain-1, ubiquitin carboxyl-terminal hydrolase 25 (USP25), aminoacylase-1, dCTP pyrophosphatase 1, 26S proteasome non-ATPase regulatory subunit 7, Xaa-Pro aminopeptidase 1, proteasome subunit alpha type-1) and few other hydrolases were found to be significantly elevated in GC-naïve DMD patients relative to controls at baseline, remained relatively stable overtime within the age range studied and did not respond to GC treatment. The gene discussed is ACY1; the disease is Duchenne muscular dystrophy.