Similarly to VSMCs, also U87MG cells, a glioblastoma cell line that expresses low levels of PDE5, up-regulated both the A1 and A3 isoforms when transfected with NOTCH3 (Fig. 3B), suggesting a common mechanism of NICD3 action on PDE5 expression in smooth muscle and neural cell types. The gene discussed is PDE5A; the disease is glioblastoma.