We found that OXPHOS was the most important metabolic pathway distinguishing T cell subtypes: CD4+ T cells exhibited significantly higher levels of OXPHOS compared to CD8+ T cells in both melanoma (GSEA p-value = 0.002) and HNSCC (GSEA p-value < 0.001, Fig. 5b, c, Supplementary Fig. 14a, b, Supplementary Data 2). This evidence concerns the gene CD4 and head and neck squamous cell carcinoma.