As shown in Fig. 6d, e, treatment of melanoma or renal cancer tumor cells with Nucl-TAP led to the activation of lentiviral transduced CD8+ T cells expressing the TCR specific to LRPAP1 peptide as measured by the acquisition of cytotoxic (Fig. 6d) and IFNγ-secreting (Fig. 6e) effector functions. The gene discussed is IFNG; the disease is melanoma.