Neither modulation of AD pathology nor microglial number grossly alter AD-related gene expression: Although we confirmed that the absence of microglia/treatment with PLX5622 did not alter the amount of Aβ produced, we wanted to explore the impact of pathology and microglia on genes associated with APP processing, Aβ clearance and metabolism, and AD in general (Fig. 5m). This evidence concerns the gene APP and Alzheimer disease.