Of the 278 missense KCNQ4 variants in gnomAD, we examined the changing amino acid residues between the transmembrane domains (TM) 5 and 6 (aa 259–296) surrounding the pore region (aa 271–292), as most known KCNQ4 variants linked to hearing loss are clustered around this region7. Here, KCNQ4 is linked to hearing loss disorder.