In the EpCAM-enriched blood of both healthy individuals and metastatic cancer patients, the relative frequencies of ldEVs over leukocytes was found to be approximately half, with one ldEV being detected for every five (in case of samples from healthy individuals, prostate cancer, and non-small cell lung cancer patients) to six (in case of samples from colorectal cancer patients) leukocytes. Here, EPCAM is linked to metastatic malignant neoplasm.