Our previous research on scanning electron microscopy imaging of CellSearch cartridges of castration-resistant prostate cancer patient samples after EpCAM enrichment [34] and the development of the open-source ACCEPT software for the automated enumeration of all fluorescently labeled objects from image data sets led to our first observations of the presence of DAPI-, CD45+, CK- objects [19]. This evidence concerns the gene EPCAM and prostate cancer.