ldEVs and leukocytes were detected in a ratio of 1:3 in the blood of healthy individuals without any pre-enrichment and in 1:5 to 1:6 in the EpCAM-enriched blood of healthy individuals and metastatic cancer patients (Figure 4), supporting the pre-existence of these ldEVs in the blood circulation instead of their formation during the EpCAM enrichment. This evidence concerns the gene EPCAM and metastatic malignant neoplasm.