Towards that direction, we enumerated ldEVs in blood samples of 10 healthy individuals without any pre-analytical or pre-enrichment steps and compared the frequencies of ldEVs and leukocytes in the whole blood to the frequency in EpCAM-enriched blood samples of 25 healthy individuals and 75 metastatic cancer patients. The gene discussed is EPCAM; the disease is metastatic malignant neoplasm.