CD40LG and infection: Of importance, the lack of TLR-stimulation also resulted in a near complete loss of CD19lo/- IgM+ CD138+ B-1PC in the MedLN at day 5 of infection (Figure 8A/B) and a corresponding drop in IgM ASC in TLR-deficient compared to control mice at that timepoint (Figure 8C), while viral loads were similarly low in the lungs of both mouse strains (not shown).