To confirm and expand these data and because these previous studies showed that only CD5+ B-1 cells could enter the MedLN, we established neonatal chimeras with varying mixes of CD5+ and CD5- B-1 cells (Figure 4A) and tested whether we could see a correlation between the frequencies of CD5+ and CD5- cells in the MedLN and/or the levels of B-1-derived IgM secretion in MedLN after influenza infection. This evidence concerns the gene CD40LG and influenza.