Colonies were cloned from the secondary cultures and analyzed by RT-PCR for mRNA expression of Gp78/AMFR, the tumor stem cell gene markers ABCG2 and Oct-4 and the thyroid differentiation markers thyroglobulin (TG) and thyroid stimulating hormone receptor (TsHR) (Figure 5A). The gene discussed is AMFR; the disease is neoplasm.